Participating Countries:

	COST is supported by the EU Framework Programme Horizon 2020
	This website is supported by COST

Liudmyla Storozhuk

Investigation of the stability, biocompatibility and cytotoxicity of iron oxide based dispersions

The goal of the work was investigation of stability, biocompatibility and cytotoxicity of the dispersion consists of magnetite NPs with hydrophobic and hydrophilic surface.

To fulfil this goal, following lines of research, are essential:

(1) Investigation of the dispersion stability based on magnetite NPs, modified with silanes in Lipiodol by determination of particles hydrodynamic diameter using dynamic light scattering DLS.

(2) Investigation of the dispersion stability based on magnetite NPs in water solution by determination of particles hydrodynamic diameter using dynamic light scattering DLS.

(3) Zeta potential measurements of obtained nanoparticles

(4) To study the cytotoxicity of obtained magnetic nanoparticles

(5) To study the interaction of obtained NPs with biological (cells, bacteria) membranes by Langmuir models.

The results obtained in this study by using DLS confirmed that the modification of Fe₃O₄ nanoparticles using silanes as a coating material led to a better dispersion of the particles in solution. All of iron oxide based oil dispersions were stable during a few hours.

In order to measure the cytotoxicity colloids of nanosized noble metals and magnetite, the cell viability of cell Human skin fibroblast line BT5ta was validated. All samples are biocompatible and have low cytotoxic action in vitro.

The interactions of the shielded magnetite NPs with bacterial mimetic membranes show a clear positive interaction between PE (E.coli lipid membrane) and shielded magnetite NPs solution.

The presence of surfactant must be mostly reduced or avoided when is not required. Then, differences based on the presence of shielded magnetite, if any, will be displayed.