print small

Participating Countries:

Australia

Austria

Belgium

Canada

Cyprus

Czech Republic

Denmark

European Commission

France

Germany

Greece

Hungary

Ireland

Israel

Italy

Norway

Poland

Portugal

Romania

Russian Federation

Serbia

Slovakia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States of America

COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST
2/8/2017 (Added to site)
Author(s): Olivier Sandre, Coralie Genevois, Eneko Garaio, Laurent Adumeau, Stéphane Mornet, Franck Couillaud

In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia

Journal: Genes
DOI: 10.3390/genes8020061

The present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and noninvasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected subcutaneously with Matrigel™ to generate so-called pseudotumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 h after heating. We showed that dextran-coated magnetic iron oxide nanoparticle dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as noninvasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release.



Founding Members

Project Office

STSM



Subscribe to newsletter